human ace 2 duoset elisa Search Results


94
R&D Systems human ace2 duoset kit
Human Ace2 Duoset Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+ace+2+duoset+elisa/Human+ACE-2+DuoSet+ELISA/pmc09135678-82-31-35
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R&D Systems human ace2 duoset elisa kit
Quantification of lung <t>ACE2</t> by <t>ELISA</t> and lung viral load by RT-PCR. (A) Lung ACE2 in Uninfected mice treated or not with Lisinopril for 21 days: Untreated Uninfected (UU ) ( n = 10), Lisinopril-treated Uninfected (LU ) ( n = 8). (B) Lung ACE2 in mice treated or not for 21 days with Lisinopril and then infected with SARS-CoV-2: Lisinopril-treated Infected ( ) ( n = 10/timepoint), Untreated Infected ( ) ( n = 10/timepoint); the mean value for Untreated Uninfected controls (UU) is shown as a dashed line ( -- - ). (C) Viral load and (D) correlation (linear regression) between viral load and ACE2 levels in Lisinopril-treated Infected mice (6/7 dpi): Lisinopril-treated Infected ( ) ( n = 10/timepoint), Untreated Infected ( ) ( n = 10/timepoint). Data presented as mean ± SEM. Asterisks indicate significance; * p < 0.05, ** p < 0.005 and *** p < 0.0005 by ANOVA 2 way with Bonferroni’s multiple comparisons and t -test.
Human Ace2 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+ace+2+duoset+elisa/Human+ACE-2+DuoSet+ELISA/pmc11282493-77-17-22
Average 93 stars, based on 1 article reviews
human ace2 duoset elisa kit - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

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Quantification of lung ACE2 by ELISA and lung viral load by RT-PCR. (A) Lung ACE2 in Uninfected mice treated or not with Lisinopril for 21 days: Untreated Uninfected (UU ) ( n = 10), Lisinopril-treated Uninfected (LU ) ( n = 8). (B) Lung ACE2 in mice treated or not for 21 days with Lisinopril and then infected with SARS-CoV-2: Lisinopril-treated Infected ( ) ( n = 10/timepoint), Untreated Infected ( ) ( n = 10/timepoint); the mean value for Untreated Uninfected controls (UU) is shown as a dashed line ( -- - ). (C) Viral load and (D) correlation (linear regression) between viral load and ACE2 levels in Lisinopril-treated Infected mice (6/7 dpi): Lisinopril-treated Infected ( ) ( n = 10/timepoint), Untreated Infected ( ) ( n = 10/timepoint). Data presented as mean ± SEM. Asterisks indicate significance; * p < 0.05, ** p < 0.005 and *** p < 0.0005 by ANOVA 2 way with Bonferroni’s multiple comparisons and t -test.

Journal: Frontiers in Pharmacology

Article Title: Lisinopril increases lung ACE2 levels and SARS-CoV-2 viral load and decreases inflammation but not disease severity in experimental COVID-19

doi: 10.3389/fphar.2024.1414406

Figure Lengend Snippet: Quantification of lung ACE2 by ELISA and lung viral load by RT-PCR. (A) Lung ACE2 in Uninfected mice treated or not with Lisinopril for 21 days: Untreated Uninfected (UU ) ( n = 10), Lisinopril-treated Uninfected (LU ) ( n = 8). (B) Lung ACE2 in mice treated or not for 21 days with Lisinopril and then infected with SARS-CoV-2: Lisinopril-treated Infected ( ) ( n = 10/timepoint), Untreated Infected ( ) ( n = 10/timepoint); the mean value for Untreated Uninfected controls (UU) is shown as a dashed line ( -- - ). (C) Viral load and (D) correlation (linear regression) between viral load and ACE2 levels in Lisinopril-treated Infected mice (6/7 dpi): Lisinopril-treated Infected ( ) ( n = 10/timepoint), Untreated Infected ( ) ( n = 10/timepoint). Data presented as mean ± SEM. Asterisks indicate significance; * p < 0.05, ** p < 0.005 and *** p < 0.0005 by ANOVA 2 way with Bonferroni’s multiple comparisons and t -test.

Article Snippet: Detection and quantification of ACE2 protein in tissues by ELISA (Enzyme-Linked Immunosorbent Assay) were performed using the Human ACE2 DuoSet ELISA kit (R&D Systems- Cat. DY933-05), following the manufacturer’s recommendations.

Techniques: Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Infection

Assessment of lung capacity through the parameters of respiratory frequency (A) , Enhanced pause (Penh) (B) , tidal volume (C) and expiratory flow curve (Rpef) (D) in K18 hACE2 mice observed on days 3 ( n = 30), 5 (n = 20) and 6/7 ( n = 10) post-infection (dpi). Data presented as mean ± SEM. Asterisks indicate significance: * p < 0.05, ** p < 0.005, *** p < 0.0005 and **** p < 0.0001 by 2-way ANOVA with multiple comparisons by Bonferroni. Representative results from three independent experiments. Untreated Uninfected ( ), Lisinopril-treated Infected ( ), Untreated Infected ( ).

Journal: Frontiers in Pharmacology

Article Title: Lisinopril increases lung ACE2 levels and SARS-CoV-2 viral load and decreases inflammation but not disease severity in experimental COVID-19

doi: 10.3389/fphar.2024.1414406

Figure Lengend Snippet: Assessment of lung capacity through the parameters of respiratory frequency (A) , Enhanced pause (Penh) (B) , tidal volume (C) and expiratory flow curve (Rpef) (D) in K18 hACE2 mice observed on days 3 ( n = 30), 5 (n = 20) and 6/7 ( n = 10) post-infection (dpi). Data presented as mean ± SEM. Asterisks indicate significance: * p < 0.05, ** p < 0.005, *** p < 0.0005 and **** p < 0.0001 by 2-way ANOVA with multiple comparisons by Bonferroni. Representative results from three independent experiments. Untreated Uninfected ( ), Lisinopril-treated Infected ( ), Untreated Infected ( ).

Article Snippet: Detection and quantification of ACE2 protein in tissues by ELISA (Enzyme-Linked Immunosorbent Assay) were performed using the Human ACE2 DuoSet ELISA kit (R&D Systems- Cat. DY933-05), following the manufacturer’s recommendations.

Techniques: Infection

(A) Body weight loss (%) and (B) clinical score (daily sum) of K18 hACE2 Untreated ( ; n = 30) and Lisinopril-treated ( ; n = 30) mice during SARS-CoV-2 infection, compared to the Untreated Uninfected ( ; n = 30) mice. The Untreated Uninfected group did not present clinical signs or weight loss. Representative results from three independent experiments. Asterisks indicate significance: * p < 0.05, ** p < 0.005, *** p < 0.0005 and **** p < 0.0001 by 2-way ANOVA followed by multiple comparisons by Bonferroni.

Journal: Frontiers in Pharmacology

Article Title: Lisinopril increases lung ACE2 levels and SARS-CoV-2 viral load and decreases inflammation but not disease severity in experimental COVID-19

doi: 10.3389/fphar.2024.1414406

Figure Lengend Snippet: (A) Body weight loss (%) and (B) clinical score (daily sum) of K18 hACE2 Untreated ( ; n = 30) and Lisinopril-treated ( ; n = 30) mice during SARS-CoV-2 infection, compared to the Untreated Uninfected ( ; n = 30) mice. The Untreated Uninfected group did not present clinical signs or weight loss. Representative results from three independent experiments. Asterisks indicate significance: * p < 0.05, ** p < 0.005, *** p < 0.0005 and **** p < 0.0001 by 2-way ANOVA followed by multiple comparisons by Bonferroni.

Article Snippet: Detection and quantification of ACE2 protein in tissues by ELISA (Enzyme-Linked Immunosorbent Assay) were performed using the Human ACE2 DuoSet ELISA kit (R&D Systems- Cat. DY933-05), following the manufacturer’s recommendations.

Techniques: Infection

(A) Survival curve of Untreated ( ; n = 18) and Lisinopril-treated ( ; n = 20) K18-hACE2 mice after infection with SARS-CoV-2; the Untreated Uninfected ( ; n = 6) did not show mortality. The survival Log-rank (Mantel–Cox) test was applied. (B–D) Histopathological analysis of lungs on 6/7 dpi. (B) histological scores of Lisinopril-treated Infected ( ; n = 10) and Untreated Infected ( ; n = 18) K18-hACE2 mice. Results obtained from the sum of the degrees of intensity. Data presented as mean ± SEM. Analysis performed by t -Test. (C) Representative histopathological images from lungs. Magnification = ×100. (D) Frequency of histopathological findings at 6/7 dpi in Lisinopril-treated Infected ( ; n = 10) and Untreated Infected ( ; n = 10) mice.

Journal: Frontiers in Pharmacology

Article Title: Lisinopril increases lung ACE2 levels and SARS-CoV-2 viral load and decreases inflammation but not disease severity in experimental COVID-19

doi: 10.3389/fphar.2024.1414406

Figure Lengend Snippet: (A) Survival curve of Untreated ( ; n = 18) and Lisinopril-treated ( ; n = 20) K18-hACE2 mice after infection with SARS-CoV-2; the Untreated Uninfected ( ; n = 6) did not show mortality. The survival Log-rank (Mantel–Cox) test was applied. (B–D) Histopathological analysis of lungs on 6/7 dpi. (B) histological scores of Lisinopril-treated Infected ( ; n = 10) and Untreated Infected ( ; n = 18) K18-hACE2 mice. Results obtained from the sum of the degrees of intensity. Data presented as mean ± SEM. Analysis performed by t -Test. (C) Representative histopathological images from lungs. Magnification = ×100. (D) Frequency of histopathological findings at 6/7 dpi in Lisinopril-treated Infected ( ; n = 10) and Untreated Infected ( ; n = 10) mice.

Article Snippet: Detection and quantification of ACE2 protein in tissues by ELISA (Enzyme-Linked Immunosorbent Assay) were performed using the Human ACE2 DuoSet ELISA kit (R&D Systems- Cat. DY933-05), following the manufacturer’s recommendations.

Techniques: Infection